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Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ).
They have established a paclitaxel resistance subline SKpac from the sensitive counterpart SKOV3 followed by quantitative proteomic analysis and further validated their findings by western blotting.
Forster et al. have previously demonstrated by quantitative proteomic analysis that the amount of collagen I, as well as α2 and α11 integrins, increases during hMSC osteogenic differentiation.
In agreement with these data, a high level of SLC16A1 expression at the protein level was detected by quantitative proteomic analysis of hCMEC/D3 cell extracts [ 19].
Indeed, Glut-1 expression was found by quantitative proteomic analysis to be 15-fold higher in hCMEC/D3 cells than in HUVECs and similar to that of human brain microvessels [ 19].
Protein expression of P-gp/MDR1, MRP4, BCRP by hCMEC/D3 cells (grown on collagen-coated dishes) was further assessed by quantitative proteomic analysis [ 19], whereas no expression of P-gp/MDR1 was detected in HUVECs, used as reference non-brain EC.
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To identify dysregulated serum proteins between ccRCC patients and control group, we compared 10 ccRCC patients' sera and 10 healthy people's sera by iTRAQ quantitative proteomic analysis.
Recently, a metabolic pathway of wheat seedling growth under hydrogen peroxide stress was revealed by iTRAQ-based quantitative proteomic analysis [ 22].
Using GA149-GFP expression, we identified several interacting proteins by affinity purification and quantitative proteomic analysis (Table 1).
To identify proteins targeted by p94 protease activity, quantitative proteomic analysis of COS7 cells expressing either p94 WT or CS was performed using the iTRAQ™ technique and 2-D LC-MALDI-MS/MS analysis.
4) QIL1 knockdown impaired MICOS assembly, resulting in the formation of a stable MICOS sub-complex that contains MIC60, MIC19 and MIC25 and lacks MIC10, MIC26 and MIC27 as demonstrated by native gels and quantitative proteomic analysis.
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by comprehensive proteomic analysis
by subsequent proteomic analysis
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by unbiased proteomic analysis
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