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Non-stained cells are imaged either by quantitative phase microscopy (QPm) [IATIA, Box Hill North, Victoria, 3129, Australia [1]] or by Mirau interferometry.
This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples.
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To the best of our knowledge, this is the first quantitative profiling of substrate deformation and wrinkling under cellular traction force achieved by the quantitative phase microscopy of digital holography.
We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts.
The activity of TRPV1 was measured both by a conventional fluorescence method to measure intracellular Ca2+ and digital holographic (DH) quantitative phase microscopy, which detects cell surface topology perturbations that follow to Ca2+ entry.
Bon, P., Maucort, G., Wattellier, B. & Monneret, S. Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.
Choi, Y., Yang, T. D., Lee, K. J. & Choi, W. Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination.
Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis.
This second modality is based on quantitative phase microscopy (QPM), implemented as a digital holographic microscope (DHM), a wide-field imaging technique which enables the measurement of the quantitative phase shifts induced by the sample [11].
Label-free characterization of ultra violet-radiation-induced changes in skin fibroblasts with Raman spectroscopy and quantitative phase microscopy.
Quantitative phase microscopy is an effective tool often used to measure spatial and temporal properties of semitransparent samples.
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