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Determination of neuronal changes was performed by quantitative morphometry of paraffin-embedded vertical sections.
Picrosirius red-stained 4 μm paraffin sections were analyzed for interstitial and perivascular fibrosis and arteriolar dimensions by quantitative morphometry of digitized images of the whole myocardial section (Aperio Technologies, Inc., CA).
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All 5 groups were processed 7 days postinjury for routine histology and the lesion sites were analyzed by quantitative morphometry for the degree of tissue damage at the lesion epicenter (Fig. 2).
In order to study retinopathy in this model, changes in retinal vasculature were analyzed by quantitative morphometry and related to retinal expression of 46 selected genes that were analyzed by microfluidic card PCR technology.
Data were analyzed by quantitative morphometry, as described previously.
Electron microscopic imaging was performed and NP uptake was assessed by quantitative morphometry.
Quantitative morphometry of MSC/UC samples revealed muscle/collagen ratios approximately 1.75× greater than SMC/UC controls at 10 weeks.
Quantitative morphometry of the lesion site showed significant tissue preservation and/or prevention of tissue decay in the unirradiated and in the irradiated group as compared with the control group which did not undergo any added surgical manipulation (Fig. 4A).
Quantitative morphometry of the lesion site showed significant (p = 0.015) degree of tissue preservation and/or tissue repair in the irradiated as compared with the unirradiated rats (Fig. 1b).
Quantitative morphometry of the lesion site in vivo (n = 22) in the 3 different groups, control, unirradiated and irradiated, shows that midline incision had beneficial effects on the course of events at the contusion site (Fig. 5).
Quantitative morphometry of transmission electron micrographs confirmed that the mitochondrial density (mitochondrial number/cytoplasmic area) was significantly lower in mnd2 mice than that in wide-type mice.
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