Sentence examples for by quantitative mRNA analysis from inspiring English sources
Each sample was reverse-transcribed using the PrimeScript RT reagent Kit (Takara, DaLian, China), followed by quantitative mRNA analysis.
Increased expression of Rorγ was confirmed by quantitative mRNA analysis in visceral adipose tissue of obese patients versus lean patients (mean values lean 7.9 ± 1.1 vs. obese 17.6 ± 2.9, Fig S1A of Supporting information).
By quantitative mRNA analysis in skeletal muscle biopsies (Fig 2A), we observed dramatic reduction of NOTCH3 expression in the proband and demonstrated a more moderate reduction of NOTCH3 expression in his father, whereas there was no relevant deregulation of NOTCH3 in his mother.
The quantitative mRNA analysis was performed in duplicate.
Quantitative mRNA analysis revealed significant loss of c-Jun mRNA in Atf2−/− neurons (Figure 8A).
By performing Western blot analysis on the two adipose tissues using antibodies specific for PPARγ, we could confirm the results obtained by quantitative mRNA expression analysis (Fig. 5B).
Herein we have shown that the genetic ablation of LepRb from the intestinal epithelium did not affect gut microbiota, AMP expression, or metabolic parameters, probably because of the lack of significant LepRb expression within the epithelium as suggested by LepRb-Cre reporter studies and quantitative mRNA expression analysis of intestinal epithelium.
Reviewer #1 also had concerns that the degree of knockdown of MELK by shMELK in the basal cell lines appears stronger than in the luminal cell lines, equivalent knockdown needs to be demonstrated by both quantitative protein and mRNA analysis.
For these studies to be strengthened, several more cell lines of each type need to be examined and equivalent knockdown needs to be demonstrated by both quantitative protein and mRNA analysis.
MUC5AC mRNA analysis by quantitative real-time PCR also confirmed increased MUC5AC mRNA levels in starved cells.
Moreover, in a subanalysis of the EMILIA trial (NCT00829166) [ 29], HER2 mRNA analysis by quantitative reverse transcriptase-polymerase chain reaction and the PIK3CA mutational status were analyzed on tumor tissues.
