Viability of tumor cells in presence of MMF was assessed by propidium iodide dsDNA-intercalation or quantitative fluorescence analysis of enzyme-catalyzed fluorescein-diacetate metabolism.
Quantitative fluorescence analysis was performed by transformation of specific staining results to pixels and by dividing the total pixel numbers by the area analysed, thus obtaining the number of pixels/μm2, using the ImageJ software (NIH, MD, USA).
Quantitative fluorescence analysis was carried out using FACSCalibur cytometer (Becton Dickinson, San Diego, CA, USA) and CellQuest software.
Quantitative fluorescence analysis was done using FACSCalibur and WinMDI software (B&D).
We sought to experimentally validate the computational results using quantitative fluorescence analysis of receptor clustering.
Plasma AGEs were measured by quantitative fluorescence spectroscopy analysis of plasma according to the method of Munch et al. (1) in a multi-mode microplate reader (Synergy 2, Biotek, Winooski, VT, USA), and results were expressed as arbitrary units (AU).
By quantitative fluorescence microscopy analysis, we demonstrate that soluble EGFP-LC3 is indeed enriched in the nucleus relative to the cytoplasm in two commonly studied cell lines, COS-7 and HeLa.
Quantitative fluorescence PCR analysis of the samples: sample DNA was extracted, and amount of Bifidobacteria and E.coli 16 s rRNA was determined by quantitative fluorescence PCR analysis using the same reaction system and conditions as establishment of the standard curve.
We estimated survival rates, cardiac function by echocardiography, blood pressure measured by indirect tail-cuff method, telomere length in heart tissue and white blood cell were evaluated by quantitative fluorescence in situ hybridization (Q-FISH) analysis.
