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The protective effects of combined ATA and AH treatment were further confirmed by quantitative detection of influenza A viruses present in the culture supernatants by ELISA (Fig. 6).
After treatment, cell death was analyzed by quantitative detection of cytoplasmic histone-associated DNA fragments using the Cell Death Detection ELISA kit (Roche).
Aimed to assess the relationship between H.Pylori and the clinicopathological features and prognosis of gastric cancer by quantitative detection of H.Pylori. 157 patients were enrolled, all patients had a record of clinicopathological parameters.
The blot was then developed with a chemiluminescence kit (ECL plus, Amersham, Germany), followed by quantitative detection of the chemiluminescence signal with a phosphorimager (Storm 840, Amersham, Germany) and analyzed with the program Image J. The experiments were carried out at 42000 rpm and at 20 °C in an XL-I analytical ultracentrifuge (Beckman-Coulter Inc).
Cell proliferation in aggregates was further assessed by quantitative detection of adenosine 5'-triphosphate (ATP), which correlates with the number of viable cells present using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Mannheim, Baden-Würtemberg, Germany) according to the manufacturer's instructions.
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The induction of cell death was assessed by the quantitative detection of histone-associated DNA fragments in the cell death detection ELISA kit (Roche, 11 544 675 001) of adherent cells.
Quantitative detection of WSSV by RT-PCR.
A similar linear relationship was observed in the quantitative detection of rotavirus by NA-SERS [11].
CD40 was measured by an ELISA kit developed for quantitative detection of sCD40 in human body fluids (Alexis© Biochemical, Bender MedSystems, Vienna, Austria).
A novel multiplexed immunochromatographic assay (ICA) based on a time-resolved chemiluminescence (CL) strategy was developed for quantitative detection of β-agonists, by utilizing ractopamine (RAC) and clenbuterol (CLE) as the models.
Gold enhancement has been used in two studies for detecting target nucleic acid in homogenous format [40, 41]; and due to the high false-positive results associated with silver enhancement [39], gold enhancement can be used for the detection of target nucleic acids in heterogenous format and can be applied for quantitative detection of iLAMP products by Au nanoprobes in iLAMP-Au-nanoprobe method.
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