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Here, by quantitative confocal analysis, we characterized the pattern of innervation of the two excitatory inputs that impinge on PCs in DBA ho-4j mice and DBA wild-type mice.
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Classical NFκB activation was measured by quantitative confocal immunofluorescence analysis of NFκB nuclear translocation [56].
Quantitative confocal image analysis was done by single cell identification using Openlab software and the relative fluorescent intensity of green, red, blue, yellow, purple, light blue and white pixels was assessed.
We observed that cell cycle arrest by hydroxyurea did not affect transfection efficacy of NLS-modified carriers which we confirmed using quantitative confocal microscopy analysis.
Successively, we measured the ER-mitochondria distance by semi-quantitative confocal microscopy analysis of volume-rendered 3D reconstructions of z-axis stacks of confocal images of ERYFP and mtRFP.
By quantitative analysis of confocal imaging, many GFP-labeled particles (87% +/− 0.1%) were found adjacent to or touching microtubules (Figure S7C).
Furthermore, fluorescently-labeled CS/HPMCP NPs showed a 2- to 4-fold improvement in the intestinal mucoadhesion and penetration compared to CS/TPP NPs as evidenced by quantitative fluorescence analysis and confocal microscopy.
Quantitative RT-PCR, confocal analysis, and ELISA were performed to evaluate IL-32 mRNA induction and IL-32 release by FLSs stimulated with TLR2 (BLP), TLR3 (poly I C), and TLR4 (lipopolysaccharide) ligands, TNF-α and IFN-γ.
The enhanced neuropil degeneration observed in Aβ42art flies was further confirmed by confocal analysis.
Islet conformation was studied by confocal analysis in consecutive slices stained for insulin and glucagon.
Next, we explored the effects of regulatory elements on protein expression by using quantitative confocal microscopy.
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