Sentence examples for by quantitative Western analysis from inspiring English sources

To quantify changes in synaptic vesicle protein expression, we compared the levels of synaptotagmin and other synaptic vesicle proteins in eyes of SV2B wild-type and knockout mice by quantitative Western analysis.

These results were then compared with those from whole cell EPR spectroscopy on the same strains, where the amount of ββ′ in each strain was determined by quantitative Western analysis.

However, we do not see an obvious correlation between P-ERK and levels of PEA3 protein expression in cancer samples, but this would likely only be revealed by more quantitative western analysis rather than the more subjective TMA analysis.

(D) Effects of the 29 active rocaglates on cFLIP protein expression by ACHN cells were individually assessed by quantitative western blot analysis.

Following expression of the reporter (UAS-GFP-312) and control (UAS-GFP) transgenes in the germline cells of the ovary using the matalpha4-GAL-VP16 driver, GFP protein and mRNA levels were measured by quantitative western blot analysis and quantitative real time PCR (RT-PCR).

Dystrophin and β-dystroglycan immunoreactivity in SDS muscle solubilates (50 µg total protein) and WGA elutes were measured by quantitative western blot analysis using the monoclonal antibodies NCL-DYS1 to dystrophin and NCL-b-DG to β-dystroglycan (Novocastra Laboratories Ltd ,Newcastle upon Tyne, UK).

To assure ourselves that the change in LC3II levels can be used as an indicator of altered autophagic activity in HEK293 cells, we treated cells with rapamycin (100nM, 4 hrs) and measured both the total LC3I and the LC3II levels by quantitative western blot analysis.

Protein expression in cultured astrocytes was determined by quantitative Western blot analysis.

Uptake of TAT-BID protein by cells was studied by quantitative Western blot analysis of cells extracts.

Protein expression levels of total Akt and phosphorylated Akt, a key effector of PI3-kinase, were measured in sciatic nerves of newborn mice by quantitative Western blot analysis.

In this experiment, the expression of plasmid-encoded SEC62-WT or SEC62D308A was evaluated by quantitative western blot analysis of the stably transfected HEK293 cell lines.