Proviral load was normalized to the amount of cellular DNA by quantitation of the human albumin gene.
These results were further corroborated by quantitation of vector genome copies and histological analysis of reporter (tdTomato) gene expression.
We have used a flow-cytometry-based assay for investigating endocytic trafficking by quantitation of polyplex pH microenvironments.
The cytotoxicity of the coatings was assessed by quantitation of growth of L-929 cells on the coatings over 72 h using lactate dehydrogenase assay (LDH).
Fijalkowski et al. (2014) detected the presence of E. coli O157 H7 in wastewater and sewage sludge in Poland by quantitation method of (EMA) real time-PCR.
Intracerebral tumor burden was monitored over time by quantitation of photon emission and tumor volume using a cryogenically cooled CCD camera and magnetic resonance imaging (MRI), respectively.
Tumor burden and tumor volume were monitored in vivo over time by quantitation of photon emission using Xenogen IVIS 50 and standard external caliper measurement, respectively.
An in vivo correlate was sought by quantitation of relaxin gene expression in 10 fetal membranes from women with normally grown and large for gestational age infants.
These results raised the possibility that patients with bladder cancer may be followed for progression or remission of their disease by quantitation of these substances in their urine.
Growth was measured by quantitation of intracellular ATP concentration by luciferase-based luminescence with a Lumitester PD-30 and LuciPac Pen (Kikkoman biochemifa, Tokyo, Japan) according to the manufacturer's instruction.
Analysis of these variants by screening for enhanced green fluorescent protein reporter expression and by quantitation of minigenomic RNA levels demonstrated that the upper portions of the putative panhandle and 3′ genomic structures can be destabilized without affecting virus replication.
