We examined the individual expression of the following genes HMGCR, FDFT1, SQLE and DHCR7 by quantifying their mRNA levels in liver cells of SUS and RES males.
Moreover, five ethylene sensing components such as CTR1, MPK6, EIN3 and two ERF1/2 were further characterized by quantifying their mRNA levels during petal senescence with real-time PCR.
For each pair of pathways, a score was derived from the distribution of gene expression levels by quantifying their pathway cross-talk.
We incubated purified O-MAD2 or C-MAD2 24 hours at 37°C, then separated them by ion-exchange and quantified their relative levels.
To determine the expression patterns of these six miRNAs, we quantified their expression levels by performing ISH assays on gastric TMA specimens.
Since two ubiquitous hyaluronidases, HYAL1 and HYAL2 were likely to account for the hyaluronidase activity, we quantified their mRNA levels by real-time RT-PCR.
A deformable template extracted the gene spots and quantified their expression levels by determining the integrated intensity of each spot after background subtraction.
Since there are currently no specific antibodies available that can distinguish between the different splice variants, we quantified their expression levels by performing a reverse transcriptase and polymerase chain reaction (RT PCR).
In order to determine whether NEUROD2 is required for the expression of cortical layer-specific transcription factors, we quantified their mRNA levels by RT-qPCR after Neurod2 knockdown in primary cortical neurons (Fig. 5d, h, l, and p).
Since the two ubiquitous hyaluronidases, HYAL1 and HYAL2, were likely to account for most or all of the hyaluronidase activity, we quantified their mRNA levels by real-time RT-PCR (figure 2).
