Since cytokine profiling is semi-quantitative, we therefore quantified the secretion of OPG by ELISA as described in the methods.
Of course on the one hand this observation emphasizes that truncated hiAP provides a powerful tool to quantify the secretion efficiency in living ciliate cells by a very sensitive assay.
As a positive control we also quantified the secretion of the IL-8 and Gro chemokines that are produced by human melanoma [ 37].
To quantify the polarized secretion of IL-6 and IL-8, supernatant samples obtained from apical or basolateral compartments were analyzed by ELISA.
ELISA and western blotting were performed to quantify the OPG secretion and measure the protein levels respectively.
By quantifying by Elisa the levels of specific IFN-γ secretion after a longer incubation period (72 hrs) of stimulation with the same pools of peptides, we were able to detect positive reactivity against many regions of the protein (Fig. 2Bii), but this response was subtype specific as it was only detected after the EnvB immunization.
We report the efficacy of MicroCT in quantifying the volume of a model of secretions in a bench study.
The slope of the relationship between quasi-steady-state insulin and glucose concentrations (or changes in concentration) quantifies the amplification of secretion typical of incretin action.
To thoroughly understand the extent of VEGF/VEGFR2 inhibition by TAM and CXB in combination, we performed VEGFR2 overexpression studies through luciferase assays and quantified the serum VEGF secretion levels.
Pulse-chase analysis was used to quantify the differences in secretion between wild-type and the four mutants of neuroserpin.
