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The extent of mineralization of lindane was determined by quantifying the release of inorganic chloride ions and analyzing the percent residual lindane in the medium.
Cell necrosis was evaluated by quantifying the release of the stable cytosolic enzyme lactic dehydrogenase (LDH), using the Cytotoxicity Detection kit from Roche Diagnostics (Meylan, France) as recommended by the manufacturer.
We assessed degranulation of mast cells by quantifying the release of β -hexosaminidase.
β-Glucosidase activity was measured by quantifying the release of pNP (p-nitrophenol) from pNP-βGlc as described previously [ 50].
Necrotic cell death was evaluated by quantifying the release of the stable cytosolic enzyme lactic dehydrogenase (LDH).
This was done by quantifying the release of Gaussia luciferase (dNGLUC) from ACTB-LC3B-dNGLUC expressed in 293T (Fig. 5 A) and HuH7 (Fig. 5 B) cell lines.
Similar(50)
Total calcium content of the mineralized substrates was determined by quantifying the Ca2+ ions released (n = 3) from the substrate upon treatment in a hydrochloric acid solution with a Thermo Scientific calcium ion selective electrode attached to a VWR Symphony pH/ISE meter.
Table 3 lists the relative activities determined by quantifying the amount of ammonia released during the hydrolysis.
This assay measures cell wall recalcitrance by quantifying the amount of monomeric sugars released during cell wall digestion [ 1- 5].
The extent of [γ-32P]-ATP hydrolysis was determined by quantifying the amount of inorganic 32P (Pi) released, which is resolved from un-hydrolyzed ATP and ADP by TLC on polyethyleneimine cellulose plate (J.T. Baker, Germany) developed with 0.5 M LiCl and 0.5 M formic acid.
Our results quantify the release process triggered by hydration of the Na2 site that occurs concomitantly with a conformational transition from an outward-facing to an inward-facing state of the transporter.
More suggestions(15)
by measuring the release
by determining the release
by quantifying the secretion
by quantifying the area
by quantifying the role
by quantifying the divergence
by quantifying the percentage
by quantifying the number
by quantifying the transcription
by quantifying the ability
by quantifying the certainty
by quantifying the model
by quantifying the entropy
by quantifying the movement
by quantifying the concept
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