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Normalization of input cDNA was performed by quantifying gene expression of stably expressed internal reference genes as described earlier (Ceelen et al., 2014; Messiaen et al., 2012).
We then investigated its capacity to accelerate naïve hMSC osteogenic differentiation by quantifying gene expression, intracellular alkaline phosphatase production, and calcium deposition when cultured on DMs or tDMs.
The aim of this study was to determine the prognostic significance of kallikrein-related peptidase 6 (KLK6) and kallikrein-related peptidase 13 (KLK13) in epithelial ovarian cancer by quantifying gene expression levels with tumour pathology and patient survival data.
We tested for both acute and chronic stress response by quantifying gene expression in fish that experienced diel water temperatures similar to desert environments that peaked near their thermal maxima (~28.5 C) over the course of four weeks.
To summarise, the data presented here support the hypothesis that at least some breast cancer patients, who develop severe reactions to radiotherapy, have an intrinsic radiosensitivity that can be identified in peripheral blood lymphocytes by quantifying gene expression response to IR and that CDKN1A is a surrogate marker for early effects of radiation therapy.
On the basis of these previously observed KLK6 and KLK13 effects on ovarian cancer cells, the aim of this study was to evaluate the prognostic significance of KLK6 and KLK13 in epithelial ovarian cancer by quantifying gene expression levels and correlating them with clinical variables and patient survival data.
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Noninvasive imaging presents a means to overcome these problems by monitoring and quantifying gene expression through the use of a reporter-based system.
The selection of most appropriate reference gene(s) is a crucial step in studies quantifying gene expression by quantitative real-time PCR (qPCR).
That is, even when a reference genome is available, using it for quantifying gene expression alone by direct mapping can be a risky, or even flawed, approach.
We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs.
TaqMan® Assays recommended by the manufacture as most efficient for quantifying gene expression from each gene were purchased from Life Technologies (Grand Island, NY).
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