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Maturation of these engineered discs was evaluated over 15 weeks in culture by quantifying cell viability and metabolic activity, construct biochemical content, MRI T2 values, and mechanical properties.
The resistant phenotype was characterized by quantifying cell viability at different concentrations of erlotinib and also via a clonogenic assay.
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We used the CellTiter 96 AQueous Kit® assay (Promega), an MTS-based assay, to quantify cell viability by measuring cellular metabolism.
This commercial kit quantified cell viability by measuring the amount of ATP released from viable cells.
To quantify cell viability by trypan blue exclusion, live cells were enumerated after trypan blue staining using light microscopy.
To quantify the cell viability, the formazan products solubilized by DMSO (Sigma, USA) were measured by spectrophotometer (Bio-Rad, USA) with 570 nm and 630 nm of wavelength.
The growth-inhibitory effects of AMF/PGI-paclitaxel conjugate and free paclitaxel was quantified by measuring cell viability using a crystal violet colorimetric assay [53].
An MTS assay was performed to quantify the cell viability.
To ensure that gene suppression was not due to CO cytotoxicity, cell viability was verified by quantifying live cells using a nucleocounter (New Brunswick Scientific, Edison, NJ).
We quantified cell numbers by counting in a hemocytometer and assessed viability by exclusion of trypan blue dye.
In order to evaluate the cytocompatibility of the PIAC-based hydrogels, the percentage of viable cells on hydrogel extracts were quantified by the MTT cell viability assay.
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