Sentence examples for by quantification using the from inspiring English sources
Band intensities were determined by scanning the exposed films followed by quantification using the Image Gauge 3.0 software (Fujifilm, Düsseldorf, Germany).
Final libraries were validated by quantification using the Qubit 2.0 fluorometer with the HS DNA quantification reagents (Invitrogen) and using the Bioanalyzer 2100 Desktop System with the DNA1000 Kit (Agilent Technologies).
Protein extraction of the micro-masses stably overexpressing FRZB or controls after seven days was performed using cell extraction buffer supplemented with 1 mM phenylmethanesulfonyl and 5% protease inhibitor cocktail, followed by quantification using the Pierce BCA Protein Assay kit (Thermo Scientific).
By semiautomatic quantification using the positive pixel count (v9) algorithm provided by the ImageScope software (version 11.1.2.760, Aperio Technologies), the staining intensity of each pixel in the TMA cores was determined.
The target gene expression levels were determined by relative quantification using the comparative CT method, also known as 2-∆∆CT method (comparative 2 [delta][delta]Ct method).
Intensity of immunoreactive bands was estimated by densitometric quantification using the Gel analyzer v1.0 software.
For both types of gene expression the target genes were quantified by relative quantification using the comparative CT method.
However, there was a significantly increase in the number of pixels with relatively higher RhoA activity at the tail of migrating cells upon EGF stimulation, evidenced by the quantification using the automated cluster analysis (Figure 6D).
Cellular growth was evaluated by protein quantification using the DC Protein Assay Kit (Bio-Rad, Hercules, Calif .. Total intracellular metal contents were quantified by inductively coupled plasma-mass spectroscopy (ICP-MS) at distinct phases of growth.
Gene expression levels were determined by relative quantification using the expression of histone H1.2 for normalization.
For qRT PCR, data were analysed by relative quantification using the standard curve method (see Applied Biosystems user bulletin 2).
