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FHL 124 cell survival was assessed by quantification of total protein content, a cell population measure.

TTR aggregation was measured by quantification of total protein precipitated, turbidity at 330 nm, and by thioflavin T (ThT) binding fluorescence.

Additionally, TNF injection in vivo resulted in a loss of goblet cells containing mucus, determined by quantification of total mucus and MUC2 immunofluorescence.

Furthermore, the most significant difference in receptor levels is seen by quantification of total ER levels expressed by all the tissue.

The control of proteins deposition was performed by quantification of total protein of each sample using the Bio-Rad protein assay kit (Bio-Rad, Munich, Germany) and staining of actin using an anti-actin antibody (1/,2000; Sigma-Aldrich, Lyon, France) (data not shown).

Similar(55)

The membrane affinity is then calculated by quantification of the total drug concentration and the amount of drug remaining in the aqueous phase after incubation with solid-supported lipid membranes.

The luciferase activities were then normalized to cell number by quantification of the total protein concentration in the samples using the Non-interfering protein assay (GBiosciences) following manufacturer's instructions.

These defects during the formation of the bronchial and respiratory tree after Hmga2-KD were validated by quantification of the total number of terminal branches and branch length.

The bacterial growth was determined by quantification of the total proteins after centrifugation of the sample using the Lowry method [ 40].

To this aim, fibrosis was assessed at 3, 7, 14, 21, and 56 days after BLM IT instillation (3 56 d) by quantification of the total cell number, percentage of surface occupied by collagen, and a modified Ashcroft score.

A time series of the images was generated and the bioluminescent intensity (BLI) was determined by quantification of the total photon emission using the LivingImage® 4.1 software package (Caliper Life Sciences).

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