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The reactions incubated at 37°C for 1 h in the dark, followed by quantification of alkaline phosphatase activity by measuring the absorbance at 405 nm.
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Quantification of alkaline phosphatase activity was performed using Amplite colorimetric alkaline phosphatase assay kit (ABD Bioquest, USA) as per manufacturer's instructions.
Quantification of alkaline phosphatase stained larvae shows significant increase in neuromast number in all mutants compared to control siblings (E, Student's t -test, p≤1.0E−44).
DNA damage was determined by quantification of γ-H2AX γ-H2AXormation and by alkaline single-cell gel electrophoresis assay (comet assay).
(A ) The rate of dephosphorylation of 4p-PTEN and its mutants was measured by quantification of bands from western blot analysis after treatment of the PTEN protein with 1 μM alkaline phosphatase.
Mitochondrial function was determined by quantification of respiratory complexes activities.
Cells RNA was standardized by quantification of GAPDH.
Without their frontman, they have been joined by Matt Skiba of Alkaline Trio and have just released their new album, California.
Osteoblastogenesis was assessed by intensity of alkaline phospatase (AP) histochemical staining.
Osteogenic differentiation was confirmed by the expression of alkaline phosphatase.
The visualization step included incubation with polyclonal rabbit anti-mouse immunoglobulins followed by preformed complexes of alkaline phosphatase/monoclonal mouse anti-alkaline phosphatase (APAAP detection system, Dako).
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