Sentence examples for by qPCR based on from inspiring English sources

MicroRNA-21 was analysed by qPCR based on tumour tissue.

MicroRNA-21 was analysed by qPCR based on FFPE tumour tissue sections.

Fourteen genes were selected for validation by qPCR based on the specificity of their top blast description and previous association with glyphosate resistance in other weed species.

Some patients did not meet standard criteria for IPA, but had consistently high levels of Aspergillus DNA in BAL fluid by qPCR based on repeated assays (Table 3).

Subsequent to microarray data analysis a set of genes was chosen for validation by qPCR based on their degree of expression change, p-value, and/or known effects of the toxin studied.

Reverse transcription was performed in 10 μL of the high-capacity master mix (Applied Biosystems, Carlsbad, CA), and duplicate reactions were analyzed by qPCR based on duplex hydrolysis probes to simultaneously measure expression levels of the target gene, BCR-ABL, and the control gene, wild-type ABL.

Fifteen regions were selected for quantitative analysis of the methylation status by quantitative PCR (qPCR) based on : 1) their distinct position on the chromosomes, 2) the presence of a CpG island within the active region, and 3) their location upstream of a gene known to be regulated by DNA methylation (although this criteria was not exclusive) (Table 1).

Among the genes higlighted as significantly deregulated in our previous microarray study [ 14], 24 genes were selected for further validation by using the quantitative Real-Time PCR (qPCR), based on their cellular function, such as transport, signal transduction, intracellular and cell surface signalling, cell cycle, replication-repair of DNA, and protein folding.

dPositive by real-time qPCR/RT-qPCR Based on multivariable regression models, in FCV-suspect animals only intact reproductive status, living in a group of at least four cats and co-infection with M. felis remained in the final model with all odds ratios being reduced by at least 10%% compared to the univariable results (Table  3).

ePositive by real-time qPCR/RT-qPCR Based on univariable regression models, in FCV-suspect cats intact reproductive status, living in a group of at least four cats, co-infection with M. felis and both vaccination and primary immunization were found to be significantly associated with FCV infection (Table  2).

Confirmation of array results by qPCR was based on eight genes (4 up- and 4 down-regulated) in the same individual samples as used for microarrays.