Sentence examples for by qPCR analysis using from inspiring English sources

mRNA expression was confirmed by qPCR analysis using SYBR green method.

Amplification efficiency of the primers was determined by qPCR analysis using serial dilutions of the cDNA template.

Endogenous gene copy numbers were determined by qPCR analysis using the ABI PRISM 7900 system with SYBR Green mix reagents (Applied Biosystems).

Differences in the abundance of the Bacteroidetes and lactobacilli were also determined by qPCR analysis using phylum and genus-specific primer sets and each of the fecal DNA pools.

This data analysis was validated by QPCR analysis using primers designed around the core promoter region at the PR1 locus using a mononucleosomal template (see Additional file 3, primer sequences cover a −22 to −147 bp region with respect to TSS).

The HNF4α binding to the Cdx-2 promoter was verified by ChIP qPCR analysis using primers covering the HNF4α positive region.

ChIP DNA was quantified by real-time qPCR analysis using a commercial master mix and thermal cycler (SYBR® Green PCR Master Mix and ABI PRISM 7500; Applied Biosystems).

Relative quantification of gene expression was performed by real-time PCR (qPCR) analysis using SYBR green chemistry.

Validation of microarray transcriptomic data was performed by quantitative RT-PCR (qPCR) analysis using the Qiagen QuantiTect SYBR Green system and a Roche Lightcycler 480.

To confirm the deletions found by MLPA, we performed a qPCR analysis using primers that were designed for selected introns and we chose three genes for normalization (GPR15 and ZIC4 on chromosome 3 and PRR15 on chromosome 7).

The heritability of expression level of selected genes with trait-associated expression levels was validated by quantitative polymerase chain reaction (qPCR) analysis using the O3 and R5 parents and a group of F1 individuals in the O3 × R5 F1 population growing in a location different from the trees used for DNA microarray analysis and sampled during a different year.