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IL1A depletion was verified by qPCR analysis in NHEKs.
Their expression was quantified by QPCR analysis in all patient samples.
DNMT1, DNMT3A, DNMT3B, TET1, TET2, TET3 and GADD45A relative expression was determined by qPCR analysis in each of the blood cell subpopulations.
The in vivo knockdown efficacy was confirmed by qPCR analysis in tumor tissues collected at the end of the experiments (Supplementary Fig S8).
PPARA mRNA levels were measured by qPCR analysis in SNU-449 cells transfected with an siRNA negative control (si-NC) and an siRNA against CDH1 (si-CDH1), 48 h post-transfection.
Here we validated by qPCR analysis in a larger group of stage I/II patients (n = 38) that the mRNA expression levels of the two genes significantly correlated with the mRNA levels of the T-cell infiltration marker CD8A.
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Initially, significant correlations between gene expression values obtained from the microarray dataset and those obtained by subsequent qPCR analysis in the first group of five patients were evaluated, as a technical validation procedure.
a S100A6 mRNA expression levels, measured by qPCR analysis, are significantly up-regulated in tumor samples of CCA patients (n = 8) compared to healthy controls (n = 4).
The use of three different reference genes has recently been shown to be sufficient for accurate normalization of results obtained by qPCR analysis for protein-coding genes in C. elegans[ 7, 8], as was previously suggested [ 9].
The increased NF- κB activities after TLR3 agonist stimulation were further confirmed by qPCR analysis, showing a higher expression in NF- κB-dependent genes IL8 and I κB α.
Studies of rats with pulmonary hypertension and right ventricular hypertrophy induced by monocrotaline showed by qPCR analysis increased levels of TGF-β1 in the right ventricle but not in the left ventricle indicating association to right ventricular hypertrophy [38].
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