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Phagosome maturation was halted by putting the samples on ice.
At the end of the lyophilization process, samples were either immediately rehydrated or stored, the latter by putting the samples within an aluminum pouch under vacuum and heat-sealing the pouch using vacuum chamber packaging machine Audionvac VMS 163 (Audion Elektro, Weesp, The Netherlands).
The MNase digestion was stopped by putting the samples on ice and adding 100 mM EDTA and 10 mM EGTA (pH 7.5).
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Thereafter the unincorporated NTPs were removed by putting the sample over an RNeasy spin column (Qiagen).
Lyophilization was performed by putting the frozen samples in the commercial lyophilizer near the condenser (Freezone Plus 6, Labconco), which reaches a temperature of −80°C, for 3.5 days.
No technical dye-swaps were done; instead possible dye-bias was controlled by putting the reference sample in the Cy3 channel for ~ half the male and ~ half the female samples and in the Cy5 channel for the other half of the samples.
After de novo assembly for each sample independently, we pooled all the unassembled reads together and performed assembly for them in order to maximize the usage of data and assemble the microbial genomes that have low frequency in each read set, but have sufficient sequence depth for assembly by putting the data of all samples together.
To access turgor pressure in the organ, the osmotic pressure of the tissue cells is required, which can be obtained by extracting cell sap (e.g. by freezing, thawing and putting the sample in a syringe) and measuring its water potential with the same approach (or with other approaches, see below).
The sample begins by putting the story of Irish wind in context.
DNA was then purified by adding 0.2 volumes of potassium acetate and putting the sample on ice for 15 min.
The observed absorbance of the sample was calculated by putting the values on a linear standard curve plotted between 200 μM to 1000 μM FeSO4.
More suggestions(15)
by diluting the samples
by incubating the samples
by treating the samples
by passing the samples
by keeping the samples
by immersing the samples
by dispersing the samples
by exposing the samples
by boiling the samples
by dividing the samples
by subjecting the samples
by comparing the samples
by centrifuging the samples
by spiking the samples
by weighing the samples
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