Sentence examples for by purification through a from inspiring English sources

The spinal cords were cut into small pieces, minced, and passed through a tissue strainer and the mononuclear infiltrate obtained by purification through a Percoll gradient [54].

The labeled probe was separated from the unincorporated nucleotides by purification through a Sephadex column as described in Sambrook et al. [ 56].

The primer sequences are listed in Additional files 2 and 3. Fly RNA was extracted with TRIzol followed by purification through a PureLink RNA Mini Kit (Invitrogen) and treated with DNase I (Thermo Scientific) to eliminate possible DNA contaminants.

CH2Cl2, when used as a reaction solvent, was dried by purification through two activated alumina purification columns.

Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA).

In modern technology, radium is separated from barium by fractional crystallization of the bromides, followed by purification through ion-exchange techniques for removal of the last 10 percent of the barium.

The product 1 was obtained as a brilliant orange oily product after the reaction of the vegetable oil with rhodamine B in the presence of EDCI and DMAP (Figure 1) followed by purification through column chromatography.

Thyroid stimulating hormone, hydrocortisone, insulin, transferrin, somatostatin, and glycyl-histidyl-lysine supplementation-dependent FRLT5 cells are a clonal cell line derived from rat normal thyroid FRLT cells by purification through successive isolation of colonies [ 21].

Positive clones were then expanded from the original liquid cultures and plasmid DNA extracted using standard alkaline lysis followed by purification through Nucleospin miniprep columns (Macherey-Nagel, Hoebdt, France).

Lin et al.[ 17] used 2 different methods for the purification of goat C1q: in one, the procedure was based on two successive precipitation steps at low ionic strength, and followed by an additional purification through a Sepharose CL-6B gel filtration column; and in the other, they used a two-step chromatography, with a BioRex 70 and a Sepharose CL-6B columns.

The distilled deionized water used was prepared in our laboratory by the glass distiller followed by purification by passing through an ion-exchanger.