Sentence examples for by purification through from inspiring English sources
CH2Cl2, when used as a reaction solvent, was dried by purification through two activated alumina purification columns.
In modern technology, radium is separated from barium by fractional crystallization of the bromides, followed by purification through ion-exchange techniques for removal of the last 10 percent of the barium.
The product 1 was obtained as a brilliant orange oily product after the reaction of the vegetable oil with rhodamine B in the presence of EDCI and DMAP (Figure 1) followed by purification through column chromatography.
The spinal cords were cut into small pieces, minced, and passed through a tissue strainer and the mononuclear infiltrate obtained by purification through a Percoll gradient [54].
Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA).
The labeled probe was separated from the unincorporated nucleotides by purification through a Sephadex column as described in Sambrook et al. [ 56].
Our previous study has shown that recombinant HP-NAP expressed in B. subtilis can be obtained in high purity by negative purification through the collection of the unbound fraction using DEAE Sephadex anion-exchange resin at pH 7.5 and 8.0 [ 26].
The distilled deionized water used was prepared in our laboratory by the glass distiller followed by purification by passing through an ion-exchanger.
The BthChi74 gene products were expressed in E. coli in an active form, followed by purification to homogeneity through hydrophobic and anion exchange chromatography (Fig. 1b).
Lin et al.[ 17] used 2 different methods for the purification of goat C1q: in one, the procedure was based on two successive precipitation steps at low ionic strength, and followed by an additional purification through a Sepharose CL-6B gel filtration column; and in the other, they used a two-step chromatography, with a BioRex 70 and a Sepharose CL-6B columns.
The culture provided 22.4 mg/L of rArtinM, which activity was determined by its one-step purification through affinity chromatography on immobilized d-mannose and glycoarray analysis.
