Sentence examples for by purification of sequence from inspiring English sources

Cycle sequencing reactions were performed using Applied Biosystems BigDye Terminator Ready Reaction v3.1 Kit, followed by purification of sequence reactions and electrophoresing using ABI Prism 3130XL Genetic Analyser (Applied Biosystems).

The sequencing reaction was conducted using the BigDye Kit, version 3.1 (Applera), and M13 universal primers (forward and reverse), followed by purification of the sequences by ethanol precipitation.

VEM involves sampling of insect vectors (in this case, whiteflies) from plants, followed by purification of viral particles and metagenomic sequencing.

Homoeologous sequences were confirmed by purification of PCR products using the QIAquick PCR purification kit (QIAGEN) and Sanger sequencing.

Purification of sequencing reactions was performed by ethanol precipitation.

A high-capacity column for affinity purification of sequence-specific DNA-binding proteins.

Sequence reads could even be obtained by purification and sequencing of sterile buffer itself.

Purification of cpDNA sequencing template by this commonly used method was extremely labor intensive.

The protocols of standard PCR reaction, purification, sequencing and sequence alignment were the same (above).

i. Confirm viral insert identity by sequencing using M13F and R primers and REDTaq polymerase, followed by gel purification and sequencing of PCR product.

This assay amplified the coding region of the entire mature protein (second and third exons) to reduce potential genomic DNA targeting, and specificity was verified by random purification and sequencing of amplicons.