Sentence examples for by pulse chase analysis from inspiring English sources

However, as revealed by pulse chase analysis (Fig. 5B), processing of 45S pre-rRNA to mature rRNA species occurred without any obvious differences between Dicer−/− and control ES cells.

By pulse chase analysis, we evaluated the stability of the mutant form as compared to wild type paxillin.

As controls, we checked that none of the four drugs inhibits translation, as judged by pulse-chase analysis (Figure S2b).

To reconcile these differences, we analyzed the effects of glycan site mutations by pulse-chase analysis in Δpep4 cells, which do not impede transport but do prevent vacuolar degradation.

Results from the steady-state experiments with the M1 mutants were confirmed by pulse-chase analysis.

We tested these constructs by transient expression in tobacco mesophyll protoplasts followed by pulse-chase analysis.

The intracellular fate of the partially functional R1116T mutant was identical to that of the original Yor1-ΔF mutant: the protein was less efficiently packaged into transport vesicles reconstituted in vitro, Yor1-ΔF670-R1116T was misfoldetecteddetected by limited proteolysis and intramolecular cross-linking, and turnover was indistinguishable from Yor1-ΔF670 Yor1-ΔF670hase analysis.

This pulse chase analysis showed that the degradation of X-nsP4 (X=Arg or Tyr) was not significantly affected by p62 knockdown (Supplementary Fig. 1e, f).

Moreover, using pulse chase analysis we saw no increase in protein degradation during cell transformation (Figure 3C).

Using pulse chase analysis it has been shown previously that CPY transport to the vacuole is delayed in Δ glo3 cells (Poon et al., 1999).

We further confirmed that overall protein levels and the stability of TLR2 are not affected by palmitoylation by performing pulse-chase analysis of FLAG-TLR2 and FLAG-TLR2-C609A FLAG-TLR2-C609A FLAG-TLR2-C609Acay of the whichdemonstratedthats was similar (see Addithenal file 2: Figure S10).