Exact(1)
The lysates were freeze-thawed four times and homogenized by pulling through a 1 ml syringe 10 times, transferred into microcentrifuge tubes and centrifuged (1200 rpm) at 4°C for 45 min. The supernatant was mixed with three volumes of Laemmli buffer [250 mM Tris HCL (pH 8.3), 8% SDS, 40% glycerol, 20% 2-mercaptoethanol, 0.04% pyronin Y], boiled for 5 min at 95°C and frozen at -20°C until blotting.
Similar(59)
It was more a moment where I listened to the song, to Shirley's voice, and realized that her band's work came from place of understanding that you can only get to by pulling through it.
Straighten thin steel wire by pulling it through a row of nails.
He hit on driving dunks, and, most impressively, tightly contested turnaround fadeaway jump shots, which didn't seem as much to go in the basket as to be pulled through by a gravitational force.
At the far end of the workshop, a young man is thinning a gold wire by pulling it through a small hole in a metal plug.
It consists in reducing tube dimensions by pulling it through a die.
By pulling hair through a slit in the band, rolling it and re-fastening its snaps, the Buntastic transforms from wristband to hairbun-enabler, allowing any woman to unleash the inner schoolmarm at a moment's notice.
His greatest secret weapon is a reverse-osmosis machine that concentrates the sap by pulling it through sensitive membranes, greatly increasing the sugar content before it even hits the boiler.
And as for the Aberdeen weather, it had pulled through as well, by the narrowest of margins, after what could oh, so easily have been a wash-out.
The park conducted a "pull-through" by pulling a train along the course to ensure proper clearance.
While demonstrating the Altair in a packed hotel conference room at Rickeys Hyatt House in Palo Alto, Calif., a MITS employee was having trouble loading the software on the machine, which was performed by pulling a paper tape through a mechanical reader to install a program.
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