Sentence examples for by pulldown from inspiring English sources

The effect of foF2 increases above the center of a cyclone can be explained by pulldown forcing, which is in agreement with classical theory of atmospheric dynamics.

The directness and phosphorylation dependency of the ADAP-Nck interaction was further probed by pulldown experiments with recombinantly expressed and purified protein.

To identify dysferlin binding partners, we carried out Liquid chromatography-Mass spectrometry (LC-MS/MS) analysis on proteins obtained by pulldown with His-myc-dysferlin from mouse skeletal muscle homogenate (Figure 1).

Nucleolin was pulled down both by hTTP alone and by PABP alone when they were each immunoprecipitated with anti-Flag antibody (Fig. 1D, lanes 2 and 3), and by pulldown of hTTP when it was co-expressed with PABP (Fig. 1D, lane 4).

We corroborated the interaction of hERK1 with H2A and H4 by pulldown of GST-hERK1 and immuno-staining of histones (Fig. 4D) and observed a FRET efficiency of almost 0.2 and 0.3 between hERK1-GFP and H4 or H2A, respectively, in the nuclei of HeLa cells, with a somewhat lower in the cytoplasm (Fig. 4C).

Contribution to binding had been evaluated by pulldown assays with an external GST TID fusion protein.

Of several candidate-binding partners identified by the pulldown assays, we confirmed direct interaction by Far Western Blotting.

To map the phosphorylation pattern of EB1, we overexpressed GST-EB1 in HeLa cells and enriched GST-EB1 from cell lysates by GST pulldown.

Here, four panning rounds in solution, followed by a pulldown with streptavidin beads, were performed.

DNA-protein complexes were then isolated by GFP pulldown and molar DNA/protein ratios were calculated from fluorescence measurements (Figure 4D).

REF52 cells expressing wt or C16/20A myc-RhoA were treated with various doses of peroxide under serum-free conditions, followed by RBD pulldown assay to detect RhoA activity.