Sentence examples for by pull down from inspiring English sources

Interestingly, 32 percent of the interactions tested could be confirmed by pull down assays.

In many cases, interaction partners for individual SH3 domains have been identified, e.g. by pull down assays.

In addition, capability of NTRC to associate with the Alr4641 protein from An4641+ cells free extracts was assessed by pull down experiments.

However, lysates treated with wild-type PTP1B followed by pull down assays with PTP1B-D181A mutant failed to bring down the Gab1.

Finally, the molecular interaction between AQP1 and Aβ was investigated by immunprecipitation of recombinant fusion proteins with distinct epitope tags coexpressed in HEK293 cells, followed by pull down with tag-specific antibodies.

Replicate aliquots of the HeLa clones with regulatable expression of HA-Dock10 cultured for 24 h in the presence and absence of dox were assayed by pull down using GST-PAK1-PBD bound beads.

Deglycosylation of SCARB2 by PNGase F and the interaction between SCARB2 and EV71 in vitro by Pull-down assay can be found in the Extended Experimental Procedures.

This was assessed by pull-down assay using a synthetic decapeptide corresponding to the C-terminal residues of the receptor as a bait.

This could explain that why the interaction between PI4KIIα and PKD identified by pull-down is quite weak; both proteins are highly dynamic in membrane trafficking and signal transduction (Balla and Balla 2006; Ellwanger and Hausser 2013).

RhoA activities were determined by pull-down of GTP-RhoA.

RhoA-GTP was measured by pull-down assay (adapted from [64], [72]).