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The authentic standard 5 was synthesized from 17β-estradiol by published procedures in 5 steps with 40% overall chemical yield.
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The proteins containing the V326N substitution were purified by published procedures and did not form amyloid fibers visible by electron microscopy (data not shown).
Subcellular fractions of yeast cells were prepared by published procedures [2,36].
Yeast spheroplasts and crude mitochondrial fraction were isolated by published procedures (Gaigg et al., 1995; Glick and Pon, 1995).
Labeled retinyl acetate was synthesized by using published procedures (31).
Islet isolation methods followed our published procedures described in detail [ 1, 24, 25, 42].
Flavin and pterin contents were determined by HPLC according to published procedures.
Isothermal oligonucleotide design, array fabrication, DNA labeling, CGH experiments, data normalization, and log2 Cy3/Cy5) copy-number ratio calculations were performed by NimbleGen according to published procedures.
F1Fo ATP synthase was purified by a published procedure (Maeda et al., 2013).
The A3 and CC7 antibodies were expressed as scFv fragment in E.coli and in small immunoprotein (SIP) format in CHO-S cells using published procedures (Borsi et al, 2002) and purified to homogeneity by protein A affinity chromatography.
As significance cutoff we chose p < 0.1, in accordance with published procedures [ 33].
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