Sentence examples for by protein separation using from inspiring English sources

Briefly, gels were lysed in denaturing Laemmli buffer followed by protein separation using SDS-PAGE.

The commonly used techniques to discover these biomarkers, also referred to as quantitative proteomics, are performed by protein separation using either two-dimensional gel electrophoresis (2DE)- or liquid chromatography (LC -based methods coupLC -basedprotein identification using methodsecoupledry (MS) [ 10].

These findings could be confirmed by high resolution protein separation using 2D-PAGE with DIGE labeling [28].

In general, the classical proteomics workflow includes protein separation using gel-based or gel free techniques followed by the identification by mass spectrometry.

Protein separation using DIGE showed an average of 991 protein spots in each group of patients.

Endotoxin was removed from the protein solutions by phase separation using Triton X-114 (Sigma-Aldrich, St . Louis MO) [49], [50].

Endotoxin was removed from the protein solutions by phase separation using Triton X-114 (Sigma-Aldrich) [49], [50].

Endotoxin was removed from the protein solutions by phase separation using Triton-X 114 (Wako Pure Chemical Industries Ltd., Osaka, Japan).

Molecular weights of the extracted proteins were determined by electrophoresis separation using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli, 1970).

Eluted protein was further purified by size-based separation using a 320 ml HiLoad 26/60 Superdex 200 pg column (GE Healthcare), to which 1.5 CV of storage buffer (25 m m HEPES/200 mM NaCl/10% glycerol, pH 7.4 at 10°C) was applied.

Cells were isolated by density gradient separation using LSM 1077 Lymphocyte separation medium and sorted for CD3+ cellsCD14− CD45RO+ cells using FACS.