Sentence examples for by protein expression changes from inspiring English sources
To investigate whether chitin-induced modulation of mRNA expression is also reflected by protein expression changes, we quantified TLR and NOD protein expression using flow cytometry.
These significant protein-protein interactions and the role of Pol β in BER or other DNA metabolic functions can clearly be impacted by protein expression changes that would disrupt complex formation.
However, from 2D SDS-PAGE data it is not possible to claim whether a change is caused by protein expression alteration or by protein posttranslational modification.
We adopted a novel approach to analyze the microarray data that extends beyond the list of differentially expressed genes and focuses on the characterization of their transcriptional regulation, which is one of the key mechanisms by which protein expression changes are controlled.
The proteome of MRSA252 was analysed by 2D-PAGE to identify protein expression changes in exponentially growing cells that were exposed to linoleic acid under the challenge conditions used for the microarray experiments.
The profound suppression of preload-triggered compensatory protein expression changes by calcineurin inhibition is astounding.
Triple negative breast cancer (TNBC) cells, which are oestrogen receptor (ER−)/progesterone receptor (PR−)/human epithelial growth receptor 2 (HER2−) and have a mesenchymal phenotype, were treated with eribulin for 7 days, followed by measurement of EMT-related gene and protein expression changes in the surviving cells by quantitative real-time PCR (qPCR) and immunoblot, respectively.
Because the ubiquitination and degradation of HIF by VHL can be inhibited by iron chelation, we used iron chelation to inhibit protein ubiquitination by VHL and analyzed the resulting protein expression changes.
In agreement with previous studies, our proteomics analysis revealed protein expression changes induced by doxycycline and/or shRNA expression (i.e., proteins that changed in shSC cells relative to uninduced cells; Supplementary Table 1).
However, that was not a comprehensive study and we reasoned that the link between the transcriptomic and proteomic observations could be more efficiently addressed by the measurement of real-time protein expression changes using metabolic labeling with high mass accuracy capillary liquid chromatography (LC) mass spectrometry (MS) analysis.
