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Such results were totally confirmed by protein expression analysis (Figure 4D) in which a decrease of cyclin D1, E, A and pRb phosphorylation and an increase of p27Kip1 in a time-dependent and glutamine-independent manner were observed.
An association between CCND1 and TP53 was already suggested by protein expression analysis [ 33].
We confirmed a reduced level of Vav3 upon transfection of Vav3 siRNA by protein expression analysis relative to the control.
Previous reports have indicated that high expression of HSP90, assessed by protein expression analysis, is associated with a poor overall prognosis in breast cancer patients [ 24].
We chose to continue with 10 genes for quantitative PCR analysis and 6 of those were also confirmed by protein expression analysis using either western blots or immunocytochemistry (Table 2).
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Determination of HER2 status can be performed either by HER2 protein expression analysis using immunohistochemistry (IHC) or HER2 gene copy number analysis.
By geographic protein expression analysis, we demonstrate that loss of RKIP expression in the tumour centre is strongly associated with both the biological and clinical behaviour of the cancer.
In rats we studied the signaling pathways of stem cells on implanted bioprosthetic tissue (photooxidized bovine pericardium (POP)), by gene and protein expression analysis.
As an alternative, we outline the use of in vivo single node perturbations – by way of mouse models with targeted inactivation of specific loci – followed by gene and protein expression analysis to gauge systems-level effects.
The addition of antibodies against proteins selected by gene and protein expression analysis either increased or decreased the proliferation rate of the glioma cell lines in vitro.
Accordingly, enhanced production of Th1-related cytokines such as IFN-γ, TNF-α, IL-2, and IL-1β has been demonstrated, both by mRNA and protein expression analysis [ 1- 5, 42, 43].
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by protein expression solubility
by protein microarray analysis
by protein expression profiling
by protein expression status
by protein sequence analysis
by protein array analysis
by protein expression level
by protein expression positivity
by protein fragmentation analysis
by protein blot analysis
by protein expression variability
by protein similarity analysis
by transcript expression analysis
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