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In order to confirm the effects of EGCG obtained by protein array analysis, the expression of 15 additional genes was also analyzed by real-time PCR.
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Seven apoptosis-related proteins were modulated in CUR- and CUR+BUS-treated cells analyzed by proteins array analysis.
Multiplex ELISA cytokine analysis was performed by Searchlight Protein Array Analysis (Pierce Biotechnology).
Tumor signaling was studied by Western blotting and reverse-phase protein array analysis.Systemic ADH-1 was associated with increased growth and activation of the PI3K (phosphatidylinositol-3 kinase -AKT pathway in A375 but not DM443 xenografts.
The amounts of IL-8 and VEGF were further measured by ELISA (Figure 4C) and the results were consistent with those of the protein array analysis (Figure 4B).
To confirm the changes in proteins observed in protein array analysis and the presence of mitochondria in the apoptosis induced by boesenbergin A, we then evaluated the protein level using western blot analysis.
reverse phase protein array analysis.
We performed a protein array analysis to identify molecules related to cancer cell metastasis that were downregulated by siFUT3 or siFUT6 using protein array (data not shown).
In order to identify the contribution of central apoptosis proteins, we performed a protein array analysis.
TAB2 and TAB3 were not substrates for the MEKK1 PHD motif in our protein array analysis.
Protein array analysis was performed according to the manufacture's instruction.
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