tagArray technology is a versatile tool for profiling protein expression on any cell surface.
With RPPA, protein lysates are denatured and immobilized to the slide, a similar approach to immunoblotting, and it offers a more quantitative approach for profiling protein expression levels.
It is based on the constitutive expression of green fluorescent protein (GFP) mediated by the bacterial protein expression vector pGFPuv (Clontech, Palo Alto, USA).
Combined gene and protein expression profilings – e.g. DNA microarrays and tissue microarrays (TMA) [ 12] – may allow easier or quicker validation of results provided by xenograft studies.
The emerging molecular classification of breast cancer is based on data obtained from gene and protein expression profilings.
Various functional genomics data from gene expression, protein expression, and metabolic profiling experiments can be visualized in the context of the reconstructed metabolic network of Chlamydomonas using either the Pathway Tools Omics Viewer [ 3] or the MapMan [ 58] tools as described above.
Fig. 3 Protein expression by Western blot.
Additionally, we analyzed Noc4l protein expression by immunofluorescence assay.
With the introduction of other high-throughput methods such as tissue-arrays (Kononen et al, 1998; Korsching et al, 2002) for the determination of distinct protein expression patterns, or gene expression-profiling (Perou et al, 2000), it will be possible to gain a more complete picture of a single tumour, allowing a more detailed analysis of mechanisms in the pathogenesis of breast cancer.
Two independent western blotting analysis confirmed that levels of protein expression were modulated consistently with gene profiling results: p21 were increased in TC-71 treated with maltonis, whereas the anti-apoptotic factor BCL-2 was diminished.
