Sentence examples for by processing the fluorescence from inspiring English sources
Localization and quantification of adhesion parameters, such as "whole cell area", "adhesion number" and "adhesion size" was achieved by processing the fluorescence images of GFP-tagged vinculin variants with ImageJ software.
Prior to measurement, clusters were separated from lower frequency components of the image (diffuse fluorescence in nuclei and intracellular autofluorescence) by processing the image with wavelet function and adding together scales 1, 2 and 3 (1, 2 and 4 pixels FWHM).
For research applications where achieving maximum accuracy and precision of individual, high-time resolution (<5 min) points is crucial, users can mitigate this behavior by modifying the integration parameters or recording the full fluorescence peak and processing the data offline.
Processing the bottom cell layer with the HiLo algorithm retains both the actin and cytoplasmic fluorescence (Fig. 1 ).
The relative intensity of fluorescence was quantified by processing images with ImageJ software (Fig. 2c).
The slides were observed under an Olympus BX50 fluorescence microscope, and the images were captured with a CCD camera (Olympus DP70) followed by processing by Image J software.
Utilizing the determined background fluorescence, we process the raw fluorescence data to obtain normalized fluorescence by first subtracting each data point with individual background fluorescence and then dividing the each subtracted fluorescence by the background fluorescence.
By analyzing the fluorescence with a spectrometer, the method can detect even small amounts of nervous system tissue on a carcass.
The downward path lit by the fluorescence of the moon seemed much shorter, and ahead the main street of the town was empty.
