Sentence examples for by processing the aCGH from inspiring English sources

We began our analyses by processing the aCGH data to account for SNP effects on probe hybridization, as previous work suggests that this will improve the specificity and sensitivity of automated CNV detection [15].

Two algorithms were subsequently used to construct CNVR maps, which were also validated by processing subsamples with additional microarray platforms (Illumina 1M-Duo BeadChip, Nimblegen 385K aCGH array) and by comparing our data with publicly available information.

The primary data processed by our model are the number of deletions and amplifications in each sample that are obtained from a pre-processing of the aCGH signals.

Finally, we pre-process the raw aCGH data by segmenting the chromosome prior to clustering.

DNA copy number anomalies were plotted by the aCGH software package v1.10.0 using the R statistical language.

The reason that the percentage of aCGH BRCA1-like tumours is so high in TNBC may be caused by the approach the aCGH BRCA1-like classifier was built.

qPCR was performed to validate 2 CNVRs (CNVR_IDs 100 and 215) detected by the aCGH experiment.

The precision was 0.866, meaning that the sequencing approach missed about 13% of the mutations discovered by the aCGH approach.

Of the 320 randomly selected patients, 90 could not be analysed with aCGH due to unavailability or low quality of tumour tissue (i.e. tumour percentage, DNA yield, quality of DNA reflected by the aCGH quality score).

Breakpoint regions were amplified by PCR, using primer pairs designed to flank the position of the deletions as predicted by the genomic locations of the aCGH probes.

In the present study, genomic DNA samples from the mucosal and invasive parts of early and advanced UGCs were prepared and subjected to gene copy-number analyses using aCGH, followed by unsupervised cluster analysis of the aCGH data.