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Protein separation (Fig. 4) was followed by processing of proteins into peptides that were subsequently introduced into the mass spectrometer for analysis.
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This class of tumor-associated antigens could potentially induce more robust immune response than antigenic epitopes generated by processing of natural, unmutated self proteins and offers best potential for successful immunotherapy.
Proteases could have a specific and controlled role during the calcification process, by either degrading proteins or regulating processing of proteins into mature forms.
In theory, the only peptides with ends that do not match these criteria, should result from host-mediated proteolytical processing (e.g. by LepB) of the parent protein prior to its tryptic digestion and thus detection of partially tryptic peptides should be indicative of host-mediated post-translational processing of proteins or incorrect assignment of a start codon.
MMP-14 may also be involved in migration of endothelial cells into the ECM, by proteolytic degradation of extracellular matrix proteins and by processing of various adhesion molecules (Galvez et al. 2001).
In addition, miRNAs can also be produced by processing introns of protein-coding genes [ 5].
Together, these studies suggest a coordinating role for MMP-8 in physiological leukocyte trafficking both in acute and chronic inflammation, either through cleavage of collagen or chemokine binding proteins [38], or by processing of bioactive molecules, such as LIX [34] to control PMN migration or longevity.
It also remains unclear whether sFasL proteins are exclusively produced by processing of full-length hFasL at the plasma membrane.
They in fact are involved in post-translational processing of protein precursors by cleaving asparagine residues in P1 position of peptide bonds [ 17].
A gradual transition between the mechanisms is noted as the molecular weight of the Precipitationte is decreased.
Based on these two sets S DG and S DT, we then extracted PIN by processing raw data of protein-protein interactions (PPI) in the i2d database.
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