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In every case the possibility of cross-contamination was kept to a minimum, with positive and negative controls included to ensure no contamination, and by processing each sample individually.
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This was achieved either by processing a parallel sample of c-Kit enriched BM cells, providing a sufficient cell number for standard gel electrophoresis, or by direct inspection of each sample using the Bioanalyzer DNA1000 assay (Methods and (Additional file 2: Figure S2)).
Just the other day, we crossed another significant threshold of the mission by processing our first sample of Mars in one of Curiosity's onboard chemistry labs.
The effect of LSP time on hardness, microhardness profile and tensile properties was investigated by processing the samples for 5, 10, 15 and 20 min at pulse energy of 230 mJ and there was found to be significant increase in tensile strength.
Polyadenylated host mRNA and bacterial rRNA were eliminated by processing the samples with MICROBEnrich and MICROB Express Bacterial mRNA Purification Kits (Ambion Inc., Austin, TX), respectively.
Quality assurance and quality control were tested by processing blank samples and standard reference material (SRM; mussel tissue SRM 2977; National Institute of Standards and Technology, Gaithersburg, MD, USA).
By processing 54 samples across multiple cell lines and biological replicates, the automated phosphopeptide enrichment platform identified a core panel of significantly KRAS-G12D regulated phosphosites common to all PDA cells (Supplementary Table 4, Supporting Information).
The US NMRCD laboratory provided laboratory support by processing samples from sentinel surveillance sites while INS-MoH fonused on processing samples from other sites in Peru.
In a transcriptomic and proteomic analysis performed using small samples of rat spinal cord, it was shown that although providing similar RNA recovery yield, the protein recovery yield can be increased by processing samples using AFD followed by protein extraction using TRIzol®, as compared to direct sample homogenization in TRIzol®[ 4].
The algorithm makes updates by processing samples in a "streaming" fashion, which requires small memory and naturally adapts to large-scale data.
Care was taken to avoid batch effects by processing samples in matched groups.
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