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To minimise the infectious risk to the laboratory personnel, all analyses were performed using aliquots of a SARS-CoV culture isolate that had been inactivated by procedures previously described [ 8].
Microarray data for ISG15 expression profiling was either normalised using the RMA procedure (Bolstad et al, 2003) (HG-U133A) or by procedures previously described in Dyrskjot et al (2005) (Eos Hu03 microarray).
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Amplification of normal and expanded alleles was obtained by PCR procedures previously described [16].
Thiol and amine p K a measurements for BSH, MeO-GlcN-Cys and Cys: Macroscopic and microscopic p Ka values were measured by adopting procedures previously described for Cys anion.
Prior to animal arrival, all rooms (walls, ceilings, floors and drains) were monitored for the presence of PEDV by PCR using sampling procedures previously described [ 10].
Preparation of samples followed the procedures previously described by our group (14).
The library data were electronically filtered based on procedures previously described by us [ 45, 84].
Mast cells were obtained by peritoneal saline washings using procedures previously described [ 21].
Categories scores were determined using procedures previously described by Maggiolo and Pavez [ 16].
The phylogenetic trees and divergence time of these primate groups were calculated according to procedures previously described by Steiper and Young (2006).
Those constituents were identified by characteristic colour changes using standard procedures previously described [ 19].
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