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The researchers compared measurement methods by probing the same type of cell using multiple techniques.
The protein levels were normalized by probing the same blots with GAPDH antibody.
Protein loading was normalized by probing the same membrane with anti-actin antibody.
The efficiency of the immunoprecipitation procedure was assessed by probing the same membrane with an anti-GSTP1-antibody (Figure 4E).
The efficiency of the immunoprecipitation procedure was assessed by probing the same membrane with an anti-HA antibody (Figure 4D).
To confirm the purity of the subcellular fractions obtained, fraction-specific proteins were assessed by probing the same filters.
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Equal loading was confirmed by re-probing the same membrane with anti-β-tubulin.
HIP expression levels were normalized to the expression of the endothelial marker vWF, by re-probing the same array with a fragment of the human vWF cDNA, as different tumors and normal tissues may contain different degrees of vascularization.
β-Actin or GAPDH served as loading control by re-probing the same membrane with an antibody (1 : 4000; Calbiochem, La Jolla, CA, USA) or probed after first stripping the membrane in a stripping buffer (100 mmol l−1 2-mercaptoethanol-22-mercaptoethanol-22-mercaptoethanol-2%at 50°C for 30 min, followed by a washing and blocking pHocedure as described above.
Because some genes were represented by multiple probes, the same genes may appear in different CCS subsets.
When the detection of variants is concentrated among cases, it is not valid to compare the resulting counts to those obtained by probing only for those same alleles among controls.
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CEO of Professional Science Editing for Scientists @ prosciediting.com