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For transmission electron microscopy analysis, embryos were prepared by previously published protocols (Ichimura et al., 2007).
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Multilocus sequence typing (MLST) was performed by following previously published protocols [ 27].
Total RNA was isolated without sRNA enrichment by adapting previously published protocols [ 33- 35].
A polymer encapsulating method was employed to produce water-soluble IO nanoparticles with carboxylic acid group by following previously published protocols.
Cyanine 5.5 (corresponding to red color) conjugated poly- l-lysine (PLL) (Mw 25000 g/mol) was prepared by following the previously published protocols.
Water samples were tested for total Enterococcus spp. [ 5] and total Bacteroidales spp. [ 14], hereafter referred to as Enterococcus and Bacteroidales respectively, by qPCR using previously published protocols [ 5, 14].
‡Rhesus cytomegalovirus (RhCMV) seroprevalence for the 2004 samples (n = 40) determined by ELISA according to previously published protocols (12 ).
Purification of E. coli apo-acyl carrier protein (apo-ACP) was accomplished by minor modification of previously published protocols.
In this study, we describe a simple and highly efficient method to construct a compact human scFv phage library, by modification of several previously published protocols [ 15, 32, 46].
Genomic DNA was extracted from the bacteria using previously published protocols by Belisle et al [31].
BmaI1 was expressed and purified by a minor modification of previously published protocols.
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