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ST80 was synthesized in our lab by previously published procedures.
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The R. ferrireducens metabolic network was reconstructed in SimPheny (Genomatica, Inc., CA) by modifying previously published procedures [ 56].
CAD activity was determined by generally following previously published procedures [ 50, 81- 83].
RV VP6-specific pre- and post-challenge antibody responses were determined by measuring levels of anti-VP6 IgG and IgA in individual sera at 1 100 and two-fold dilution series by ELISA according to previously published procedures [ 20, 21].
The abstracted hospital records of cases were reviewed and adjudicated for IS subtype by a pair of neurologists according to previously published procedures (Johnson et al. 1995; Kittner et al. 1998), with disagreements resolved by a third neurologist.
Samples were assayed by inductive-coupled plasma mass spectrometry according to our previously published procedures [35].
Silicalite nanocrystals were synthesized according to the previously published procedures [15].
Moreover, it gives better results than previously published procedures (removal, arthrodesis and hemiarthroplasty of the DRUJ).
Three-dimensional FE models were established using experimentally estimated PMMA material properties (elastic and damping) from previously published procedures.
Experiments followed previously published procedures [3], [11], [12], [13], [14].
Experiments followed previously published procedures [15], [16], [17], [18].
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