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Linearity was checked by preparing standard solutions at five different concentration levels of each of FEX and its four related compounds ranging from 0.1-50 0.1-50using a fixed concentration of 30 μg/ml LIS as internal standard.
The effect of the initial Cr VI) concentrations on the adsorption was examined by preparing standard solutions of Cr VI) in the interval 0.1 1.0 mM Cr VI) solution.
Therefore, we addressed this issue retrospectively by preparing standard solutions of Muc5ac and Muc5b to characterise further the polyclonal antisera.
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Extraction efficiency from wipe media was tested by preparing control standard solutions as follows: two filter papers were wetted with 250 μL of the desorbing solvent and spiked with appropriate aliquots of antineoplastic drug stock solutions at specific concentrations assuming quantitative extraction.
The calibration curve was obtained by preparing the standard solutions of a different concentration of a range of 1 50 ppm followed by analysis using a spectrophotometer.
The preparation of adsorbate was carried out by preparing a standard solution of 2 ppm fluoride.
Linearity was evaluated by preparing a standard solution of concentration 0.1mg/mL and different volume of this solution was applied to get the required range of 200, 400, 600, 800, 1000 and 1200ng/spot.
If proceeding with stock standard B, for future analysis note that the stock standard preparation procedure can be modified by preparing a stock standard solution of 0.1 mg/mL to save one dilution step.
The linearity was determined by preparing a stock solution of pure standards of the pesticides studied and diluting them to produce a concentration range.
The solution was prepared by accurately adding appropriate volume of each of the prepared standard solution.
This was evaluated by measuring absorbance of freshly prepared standard and sample solutions, and repeating the measurement after 24 h.
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