Sentence examples for by preparing a solution from inspiring English sources

The thin fibers were produced by preparing a solution of 0.1 mg/ml fibrinogen and 0.1 U/ml thrombin 1 hour in advance and then allowing it to react with the gel for 1.5 hours.

Each secretome sample was processed, by preparing a solution of 1  μg/ μL of proteins and 40 mM of ammonium hydrogen carbonate (Sigma-Aldrich, USA).

Procedures: Film formation was achieved by preparing a solution of PIM-Trip-TB (0.50 g) in chloroform (20 mL) which was poured into a 9 cm circular Teflon mould.

The deprotected N-terminus was acylated by preparing a solution of bromoacetic acid (90 μmol) and DIC (90 μmol) in DMF (500 μL) to activate the bromoacid (2 min, RT), transferring the activated bromoacid (500 μL) to the resin and incubating (15 min, 37 °C).

The deprotected N-terminus was acylated by preparing a solution of bromoacetic acid (9.6 mmol) and DIC (4.8 mmol) in DMF (6 mL) to activate the bromoacid (2 min, RT), transferring the activated bromoacid to the resin and incubating (15 min, 37 °C).

This was done by preparing a solution of concentrated red dye (0.05 mL of DMSO with 0.0015 g of Tetramethylrhodamin-5-isothiocyanate; T0820-5MG by Sigma-Aldrich), which was homogenized in PBS to make the diluted dye (1 μL of concentrated solution with 10 mL of phosphate buffered saline).

Linearity was evaluated by preparing a standard solution of concentration 0.1mg/mL and different volume of this solution was applied to get the required range of 200, 400, 600, 800, 1000 and 1200ng/spot.

The preparation of adsorbate was carried out by preparing a standard solution of 2 ppm fluoride.

A calibration curve for NO was obtained by preparing a saturated solution of NO as described previously by Mesároš et al. [35].

The linearity was determined by preparing a stock solution of pure standards of the pesticides studied and diluting them to produce a concentration range.

Flavonolignan stability was assessed by preparing a stock solution containing 100 ppm of each flavonolignans in methanol and was stored at room temperature for 72 h.