Sentence examples for by preparing a single from inspiring English sources

14 The Mab-IFA was optimized for ease of use by preparing a single mixture of primary and secondary antibody, which was added to the slide in a single step.

To adequately determine the effects of combination, the slices were harvested 24 h later (when the effects of single-agent AdsTRAIL were minimal) by preparing a single-cell suspension from the slices and analyzing the cells for the sub-G1 (apoptotic) fraction.

Briefly, all six pairs of mammary glands from both virigin and uniparous (one-time pregnant) Lewis rats were processed by mechanical and enzymatic dissociation to prepare a single-cell suspension.

The emission spectra of each fluorophore/IHC stain was computationally unmixed by preparing matched single stained samples.

Lymphoma cell lines were generated by preparing single-cell suspensions from spleen, which were cultured at 37 °C in a humidified 10% CO2 incubator in high-glucose Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (JRH Biosciences, Brooklyn, VIC, Australia), 50  μM 2-mercaptoethanol (Sigma) and 100  μM asparagine (Sigma) referred to as FMA.

The films were prepared by a single spin coating process using mixed silica titania sols, followed by annealing at 180 °C.

The Unscear document he refers to was prepared by a single lead consultant with a handful of supporting individuals and the document was approved by nominees of the states which are subscribing members of Unscear – mostly those countries with an investment in nuclear power.

This multifunctional polymeric vehicle was prepared by a single emulsion evaporation method, using carboxyl-terminated PLGA.

Chitosan/poly(caprolactone) (CS/PCL) nanofibrous scaffold was prepared by a single step electrospinning technique.

Glass ceramic Li2S GeS2 P2S5 electrolytes were prepared by a single step ball milling (SSBM) process.

The second was that the polymer-lipid hybrid NPs were prepared by a single step which combined the nanoprecipitation (or emulsion) and self-assembly method[14, 15].