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The enantiomeric fractions of 1 were obtained by preparative enantioselective HPLC.
Final purification of hAPP-TM was achieved by preparative reversed-phase high performance liquid chromatography (HPLC).
Briefly, crude eDNA was isolated from ~0.5 kg of soil as outlined above, and further purified by preparative agarose gel electrophoresis to yield pure high-molecular-weight eDNA.
Fractions with various molar masses for each modification stage were obtained by preparative fractionation.
4-VC was purified by preparative RP-HPLC.
The Rh protein was purified to homogeneity by hydroxylapatite chromatography followed by preparative NaDodSO4/PAGE.
Purification by preparative RP-HPLC resulted in similar spleen and liver accumulation.
Total oil extracts were separated into their saturate and aromatic fractions by preparative silica gel chromatography.
It was then purified by preparative reversed-phase high-performance liquid chromatography (RP-HPLC).
Fraction B8-5 was purified by preparative HPLC to yield 4 (3.5 mg).
Compound 9 (5 mg) was isolated from fraction A1 by preparative TLC (CHCl3 MeOH, 30:1).
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