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Functional divergence among the subfamilies of the soybean expansin superfamily was inferred by posterior analysis using the program DIVERGE v2.0.
To answer this question, we estimated Type-I functional divergence between gene clusters of the grape subtilase family by posterior analysis using the program DIVERGE [ 54].
Two types of functional divergence (Type-I and Type-II) between gene clusters of the NIP subfamily were estimated by posterior analysis using DIVERGE2 that evaluates shifted evolutionary rate and altered amino acid property after gene duplication [ 54, 55].
Type-I functional divergence between gene clusters of the OPT family was estimated by posterior analysis using the program DIVERGE [ 59, 60], which evaluate the shifted evolutionary rate and altered amino acid properties.
Type I functional divergence (shifted evolutionary rate) and Type II functional divergence (altered amino acid physicochemical property) between gene clusters of the WRKY gene family were estimated by posterior analysis using the program DIVERGE v2.0 [ 66, 67].
Two types of functional divergence (type-I and type-II) between gene clusters of the OPR family were estimated by posterior analysis using the DIVERGE v2.0 program [ 36], which evaluates the shifted evolutionary rate and altered amino acid properties after gene duplication [ 37, 38].
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An ANOVA was then performed for each cannabinoid with location as a factor and a posterior post-hoc analysis using Tukey, except for THC-V where we used Bonferroni (Fig. 1).
In order to verify the topology of the Bayesian phylogenetic trees supported by the posterior probability, we performed bootstrap analysis using the Maximum Likelihood method implemented in RAxML software [ 72].
Evaluation of the PK/PD model for 4βHC The posterior predictive performance of the PK/PD model was evaluated by generating predictions for the 34 subjects included in the analysis using 500 of the posterior sets of population mean parameter estimates.
Additionally, we repeated the analysis using Krox20 as a marker for r3+r5 to clearly demonstrate the posterior expansion of Otx2 expression and lack of posterior hindbrain truncation.
Analysis using GEMMA (v. 0.94beta), which used a Bayesian method, achieved nearly identical results (posterior mode 0.83, SE 0.41).
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