Sentence examples for by positive sorting using from inspiring English sources

The plasmacytoid DCs were obtained by positive sorting using anti BDCA-4 conjugated manti BDCA-4 conjugatedltenyi Biotec, Auburn, CA).

Monocytes were purified by positive sorting using anti-CD14-conjugated magnetic microbeads and by two runs through LS columns (both from Miltenyi Biotech) to obtain purities over 99%.

Endothelial cells were cultured and purified over a 2- to 3-week period by a series of magnetic immunosortings with Dynabeads (Invitrogen), including a single negative sort using antibodies to the Fc III/II receptor (Abd Serotec) to remove macrophages, followed by at least two positive sorts using antibodies to ICAM-2 (Abd Serotec) to enrich for the endothelial cell population.

In some cases, total RNA was extracted from either CD4 or CD8 T-cells enriched by positive magnetic sorting using an AutoMACS (Milteny Biotec).

The selected pool was enriched for GFP+ cells by subsequent GFP positive cell sorting using a Coulter EPICS™ -ESP cell sorter (Beckman-Coulter, Fl) using the same filter set as described earlier.

Magnetic labelling and separation was used for the isolation of LECs from lymphangiomas by positive sorting with CD31 paramagnetic micro-beads, if the cultures were contaminated with fibroblasts.

Monocytes were isolated from PBMC by positive immunomagnetic cell sorting using CD14 Microbeads (Miltenyi Biotec, Auburn, CA) which yielded >90% purity when assayed by flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA) using anti-CD14-FITC (BD Pharmingen, San Diego, CA).

Cells stably transfected with enhanced green fluorescent protein (eGFP) were further enriched for eGFP-positive populations by sorting using a BD FACSAria flow cytometer (BD Biosciences) equipped with a 488 nm argon laser.

GDTc were enriched from the PBMC fraction by magnetic sorting using the Miltenyi γδ T cell positive selection kit (Miltenyi Biotec, Auburn CA) as per the manufacturer's instructions.

GFP positive population was purified by high speed cell sorting using FACS Aria (Becton, Dickinson).

Active sorting using acoustic forces can be improved by combining it with passive sorting methods.