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Stable dual‐gene expression was confirmed in both T‐cell types, permitting enrichment by positive selection with Rituxan.
B cells were purified from splenocytes by positive selection with CD19 MicroBeads (Miltenyi Biotec) in accordance with the manufacturer's instructions.
CD14+ monocytes were isolated from PBMCs by positive selection with CD14 microbeads (Miltenyi Biotec).
Neutrophils were obtained by positive selection with anti-CD16 coated microbeads.
DCs were purified from collagenase-trated spleens by positive selection with anti-CD11c microbeads (Miltenyi Biotech).
pDCs were enriched by positive selection with PDCA-1 conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) according to manufacturer.
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It seems reasonable to suggest that the retention of the tetraparalogous salmon Aqp0 channels may be caused by positive selection associated with the neofunctionalization of pH sensitivities to encompass a broad range of aquatic conditions.
Genome-wide scans for selection have provided long lists of genes putatively targeted by positive selection, together with information about the genomic regions and biological functions most likely to have played a role in our adaptation to the environment (reviewed by (Akey 2009)).
DCs were further enriched by positive selection of DCs with CD11c beads (Miltenyi Biotec), labeled with CD8α-PE-Cy7 (Ly-2) and CD11b-FITC (M1/70), and sorted by a FACSVantage instrument cell sorter (BD Biosciences) to complete purification of the DCs into subsets.
Thymocytes with the appropriate self-reactive signaling-module avidity survive by positive selection, and thymocytes with strong avidity are generally eliminated by negative selection.
Previous work proposed that a subfamily of A. thaliana FBX genes (mostly from STSP and FBX pseudogene groups base on our analysis (Figure 5)) evolved by positive selection (Ka/Ks >1), with variations in a collection of amino acids in the recruitment module potentially playing an important role in innate immunity and stress responses [14].
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