Seven CD19-selected sporadic CLL samples were obtained by positive selection using magnetic beads conjugated to anti-CD19 (MACS, Miltenyi Biotec, Auburn, CA).
CD34+ cells were purified by positive selection using anti-CD34 microbeads (Miltenyi Biotech, Madrid, Spain).
CD14+ monocytes were isolated by positive selection using magnetic beads (Miltenyi Biotec, Bisley, UK).
Monocytes were purified by positive selection using CD14 beads (Miltenyi Biotech, Surrey, UK).
CD34+ cells were then isolated from the MNC population by positive selection using immunomagnetic cell separation procedures.
We isolated CD14+ monocytes from PBMCs by positive selection using CD14-specific immunomagnetic beads (Miltenyi Biotech, Auburn, CA).
CD14+ cells were isolated by positive selection using magnetic beads (Miltenyi Biotech; Auburn, CA) according to the manufacturers protocol.
Monocytes were isolated by positive selection using CD14 microbeads and a magnetic cell separator (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany).
CD14+ monocytes were purified by positive selection using anti-CD14 monoclonal antibody-coated magnetic microbeads (Miltenyi Biotech, Auburn, CA) as described previously [53].
CD14+ monocytes were isolated from PBMCs by positive selection using MACS anti-CD14 microbeads and a Midi-MACS® magnetic cell sorting device (Miltenyi Biotec, Bergisch- Gladbach,Germany).
CD4+ T cells were further purified by positive selection using anti-hCD4 magnetic microbeads (1∶20 dilution) and MACS column (Miltenyi Biotech), following the manufacturer's protocols.
